Mutational analysis of 6-aminohexanoate-dimer hydrolase: Relationship between nylon oligomer hydrolytic and esterolytic activities
نویسندگان
چکیده
منابع مشابه
Nylon oligomer degradation gene, nylC, on plasmid pOAD2 from a Flavobacterium strain encodes endo-type 6-aminohexanoate oligomer hydrolase: purification and characterization of the nylC gene product.
A new type of nylon oligomer degradation enzyme (EIII) was purified from an Escherichia coli clone harboring the EIII gene (nylC). This enzyme hydrolyzed the linear trimer, tetramer, and pentamer of 6-aminohexanoate by an endo-type reaction, and this specificity is different from that of the EI (nylA gene product) and EII (nylB gene product). Amino acid sequencing and sodium dodecyl sulfate-pol...
متن کاملA plasmid encoding enzymes for nylon oligomer degradation: nucleotide sequence and analysis of pOAD2.
The entire nucleotide sequence of nylon oligomer degradative plasmid pOAD2 from Flavobacterium sp. KI723T1 was determined. pOAD2 comprises 45519 bp, with a 66.6 mol% G+C content. The precise loci of the four nylon oligomer degradation genes, namely nylA (6-aminohexanoate-cyclic-dimer hydrolase gene), nylB (6-aminohexanoate-dimer hydrolase), nylB' (a gene having 88% homology to nylB) and nylC (e...
متن کاملEmergence of nylon oligomer degradation enzymes in Pseudomonas aeruginosa PAO through experimental evolution.
Through selective cultivation with 6-aminohexanoate linear dimer, a by-product of nylon-6 manufacture, as the sole source of carbon and nitrogen, Pseudomonas aeruginosa PAO, which initially has no enzyme activity to degrade this xenobiotic compound, was successfully expanded in its metabolic ability. Two new enzyme activities, 6-aminohexanoate cyclic dimer hydrolase and 6-aminohexanoate dimer h...
متن کاملHigh homology between 6-aminohexanoate-cyclic-dimer hydrolases of Flavobacterium and Pseudomonas strains.
The nucleotide sequences of the genes for 6-aminohexanoate-cyclic-dimer hydrolases of Flavobacterium sp. strain K172 (F-nylA) and Pseudomonas sp. NK87 (P-nylA), enzymes essential for the degradation of a by-product of the nylon-6 industry, were obtained by the dideoxynucleotide chain-termination method. A 1,479-base-pair open reading frame starting at a GTG and terminating at a TGA was found fo...
متن کاملThe three-dimensional structure of nylon hydrolase and the mechanism of nylon-6 hydrolysis*
From the Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, Hyogo 671-2280; Department of Life Science, Graduate School of Life Science, University of Hyogo, Hyogo 678-1297; RIKEN Harima Institute, SPring-8 Center, Hyogo 679-5148; Institute for Protein Research, Osaka University, Osaka 565-0871, Department of Intelligence Mechanical Technology, G...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2006
ISSN: 0014-5793
DOI: 10.1016/j.febslet.2006.08.031